海洋科学Issue(12)：29-33,5.DOI:10.11759/hykx20130804001
鲜浒苔稀硫酸水解产糖工艺研究
The study of dilute sulfuric acid hydrolysis process of fresh Ulva prolifera for reducing sugars yield
摘要
Abstract
Based on the improved two-stage sulfuric acid hydrolysis method, the total sugar content of Ulva prolif-era was determined as 67.2%. The samples of fresh U. prolifera were mixed with gradient dilute sulfuric acid (0.6、1.0、1.4、1.8、2.2%(w/w) ) and hydrolyzed at 121℃with reaction time of 30, 60 and 90 min, the solid U. prolifera residues recovered after sulfuric acid hydrolysis were hydrolyzed by cellulases, the yield of the reducing sugars from each step was determined by DNS method. It was showed that in the process of acid hydrolysis, the highest conversion rate of reducing sugars was obtained as 59.9%when the concentration of the sulfuric acid was 1.8%and the hydrolyzing time was 90 min, the yield of reducing sugars was 447.0 mg every 1000 mg dry sample. However, in the process of enzymatic hydrolysis, the conversion rate of reducing sugars from residue was low, the maxium value was only 6.5%when the concentration of sulfuric acid was 0.6%and the hydrolyzing time was 60 min. It was revealed that the acid hydrolysis was effective to hydrolyze U. prolifera sugars independently, and the optimized condition in this experiment was 1.8%(w/w) sulfuric acid with 90 min of hydrolyzing time, this will improve the further research on transferring the U. prolifera as a potential material to produce fuel ethanol.关键词
浒苔/糖化工艺/酸水解/酶水解Key words
Ulva prolifera/saccharification/acid hydrolysis/enzymatic hydrolysis分类
生物科学引用本文复制引用
冯大伟,姜鹏,李富超,秦松,赵瑾,陈华新..鲜浒苔稀硫酸水解产糖工艺研究[J].海洋科学,2014,(12):29-33,5.基金项目
海洋公益项目(201205010) （201205010）
国家自然科学基金项目(3130-0365) （3130-0365）
青岛市科技项目(13-1-4-259-jch) （13-1-4-259-jch）
海洋先导专项(XDA1102-0304, XDA11020303) （XDA1102-0304, XDA11020303）
