海洋科学Issue(1)：8-12,5.DOI:10.11759/hykx20140109001
甲基磺酸乙酯对小新月菱形藻的生物学效应
The biological effect of the Ethyl Methanesulphonate on Nitzschia closterium
摘要
Abstract
In order to obtain the high-lipid producing strains of Nitzschia closterium, the method of chemical muta-tion with Ethyl Methanesulphonate (EMS) was used, and the biological effects of EMS on the cells of N. closterium were studied. The results showed that the EMS has good mutagenesis effect on the cells of N. closterium, and two different mutant strains of N. closterium YA and YB were obtained after treatment with 0.1 mol/L and 0.05 mol/L dos-ages of EMS, respectively, which both had stronger oil production capacity. Under the same cultivation condition, the chlorophyll a, carotenoids, protein, soluble sugar contents and lipid relative contents of YA and YB have in-creased by 30.05% and 25.77%, 22.15% and 19.26%, 9.06% and 8.27%, 48.25% and 45.48%, and 44.06% and 12.86%compared with the CK, respectively.关键词
微藻/小新月菱形藻(Nitzschia closterium)/诱变/甲基磺酸乙酯/油脂Key words
Microalgae/Nitzschia closterium/Mutagenesis/Ethyl Methanesulphonate/lipid分类
农业科技引用本文复制引用
杨茂纯,赵耕毛,王长海..甲基磺酸乙酯对小新月菱形藻的生物学效应[J].海洋科学,2015,(1):8-12,5.基金项目
国家863计划项目(2012AA021706) （2012AA021706）
