海洋科学Issue(1)：29-38,10.DOI:10.11759/hykx20140304001
牙鲆耐寒相关基因CIRP、HMGB1的克隆及表达特征分析
Cloning and expression analysis of cold-tolerance related genes, CIRP and HMGB1, in Paralichthys olivaceus
摘要
Abstract
In order to learn the expression patterns of CIRP and HMGB1 genes and provide more candidate genes for cold-resistant marker assistance breeding of flatfish, these two cold-tolerance related genes in olive flounder (Paralichthys olivaceus) were cloned using 5¢and 3¢RACE methods. The open reading frames of flounder CIRP and HMGB1 were 932 bp (GenBank accession number, KC735176) and 1222 bp (GenBank accession number, KC422724), respectively. The expression patterns of those two genes in flounder tissues were studied using RT-PCR analysis. The expression levels of HMGB1 were higher in liver, heart, muscle and, intestines than those in brain and gill. Under the low water temperature, expression levels of CIRP and HMGB1 genes in flounder brain, liver and muscle tissues were also studied using real-time RT-PCR method. In muscle, the expression levels of CIRP and HMGB1 increased during 0~12 h, and then decreased with the prolonging of treatment time. The expression level of HMGB1 in brain tissue increased during 0~2 h, and then decreased to the initial level, and there was no difference in expression levels in the liver tissue during the whole cold treatment process. The expression levels of CIRP in liver tissue increased during 0~2 h, and then decreased to the initial level. But there was no obvious fluctuation in brain tissue. According to the above results, we speculate that CIRP and HMGB1 could participate in flounder cold tol-eration and may promote inflammatory responses under low temperatures, so both genes could be candidate genes for cold-resistant molecular marker selection of flounder.关键词
牙鲆(Paralichthys olivaceus)/低温胁迫/CIRP和HMGB1/克隆/RT-PCRKey words
Paralichthys olivaceus/cold treatment/CIRP and HMGB1/RT-PCR分类
生物科学引用本文复制引用
胡金伟,尤锋,王倩,王丽娟,翁申达,辛梦娇..牙鲆耐寒相关基因CIRP、HMGB1的克隆及表达特征分析[J].海洋科学,2015,(1):29-38,10.基金项目
国家863计划资助项目(2012AA10A408,2012AA092203) （2012AA10A408,2012AA092203）
国家973计划资助项目(2010CB126304) （2010CB126304）
国家科技基础条件平台建设运行项目(2006DKA30470017) （2006DKA30470017）
