海洋科学Issue(1)：39-45,7.DOI:10.11759/hykx20130603002
基于线粒体COI和16S rRNA基因研究3个地理群体黑龙江河蓝蛤的遗传多样性
The genetic diversity of three populations of Potamocorbula amurensis based on mitochondrial COI and 16S rRNA gene
摘要
Abstract
Mitochondrial COI and 16S rRNA gene fragments of three wild populations Potamocorbula amurensis were amplified with universal primers; the PCR products were sequenced, and 658 bp and 450 bp nucleotide se-quences were obtained. The T, C, A, G and A+T contents in the fragment (COI/16S rRNA) were 45.4%/32.0%, 13.5%/13.3%, 20.7%/29.3%, 20.4%/25.3%, and 66.1%/61.3%, respectively. The AT content was higher than the GC content. The phenomenon was similar to the results of other mollusca. 24 haplotypes and 43 polymorphisms were defined among COI from three populations. Nine (9) haplotypes and 19 polymorphisms were defined among 16S rRNA from three populations. The fixation indices (Fst) analyzed by AMOVA were totaled to Fst=0.0.0090 (P<0.001) and Fst=0.0674 (P<0.001), showing that the genetic fixation intra-populations was larger than inter-populations. It indicated that significant genetic fixation existed among the three populations. The Neighbor-joining (NJ) phy-logenetic trees were made from the three populations of P. amurensis, showing that the three populations might have originated from one clade, while a small number of individuals may be mixed in otherclades.关键词
黑龙江河蓝蛤(Potamocorbula amurensis)/线粒体/16S rRNA/COI/遗传分化Key words
Potamocorbula amurensis/16S rRNA/COI/Genetic diversity分类
农业科技引用本文复制引用
孙超,刘志鸿,杨爱国,周丽青,吴彪,严加坤,侯丫,董春光..基于线粒体COI和16S rRNA基因研究3个地理群体黑龙江河蓝蛤的遗传多样性[J].海洋科学,2015,(1):39-45,7.基金项目
国家科技基础条件平台项目 （）
国家科技部基础工作专项(2013FY110700) （2013FY110700）
