海洋科学Issue(3)：12-18,7.DOI:10.11759/hykx20130930001
条斑紫菜SSU中内含子分析
Analysis of intron in SSU of Porphyra yezoensis
摘要
Abstract
Fragments were amplified using primers designed on the basis of SSU and LSU rRNA gene sequences of Porphyra yezoensis, respectively published in GenBank. Genomic DNA and cDNA of mixed or single Porphyra yezoensis thallus were used as templates. After sequencing and analyzing these fragments, it is presumed that the intron in SSU rRNA gene is not excised in the succedent processing after transcription, and intron was not discov-ered in LSU rRNA gene. The intron which locates upstream in SSU rRNA gene is 511 bp. The location of intron is the TCTGGTG-CCAGCAGCC exon sequence, and the 5′-end and 3′-end of the intron are AAC- and -ATGG, re-spectively. The splicing site and conserved regions P, R and S of it are different from type I intron. Moreover, there are differences in characteristics such as presence, location and structural feature of introns between individuals of Porphyra yezoensis thallus. So it is not appropriate to use introns for classification between intra-species. However, the length and nucleotide sequence of introns are specific in various species, which may provide references for dis-crimination of red algae between inter-species and intra-species with distant relationship or different biogeography.关键词
条斑紫菜(Porphyra yezoensis)/rRNA基因/内含子/同质性/系统发育Key words
Porphyra yezoensis/ribosomal DNA/introns/homogeneity/phylogenetic development分类
生物科学引用本文复制引用
李星辰,徐佳杰,林中姮,沈颂东..条斑紫菜SSU中内含子分析[J].海洋科学,2015,(3):12-18,7.基金项目
国家自然科学基金资助项目(41276134) （41276134）
