海洋科学Issue(3)：19-25,7.DOI:10.11759/hykx20140125001
虾夷扇贝不同性别类型2个Dmrt基因DM结构域分析
Sequence analysis of DM domain in two Dmrt genes of three sex types of Patinopecten yessoensis
摘要
Abstract
In order to study the molecular mechanism of sex determination and the causes of formation of her-maphrodite scallops in cultured Patinopecten yessoensis populations, we used degenerate PCR to amplify the Dmrt gene conserved DM domaln from three sex types of genomic DNA. The PCR products have a length of 141 bp, and after DNA cloning and sequencing analysis, 2 different DM domaln sequences were identified and named as pyDmrt3 and pyDmrt4. pyDmrt3 existed in three sex types, while pyDmrt4 was only cloned from male scallops ge-nomic DNA. Differences presented in Dmrt gene sequences of different sex types. Comparing to other mollusks, the DM domaln is evolutionarily conserved, while the highest variation frequency of the DNA and amino acid se-quences was in male pyDmrt3, secondly in hermaphrodite pyDmrt3, but relatively high degree of sequence homol-ogy in female DM domaln. It is inferred that part of males might come from hermaphrodite sex reversal, and the higher degree variation frequency may be one feature of hermaphrodite scallops. PyDmrt4 participates in the male sex determination possibly.关键词
虾夷扇贝(Patinopecten yessoensis)/性别/DM结构域/Dmrt基因/性逆转Key words
Patinopecten yessoensis/sex/DM domain/Dmrt gene/sex reversal分类
生物科学引用本文复制引用
周丽青,杨爱国,王清印,郑利兵,刘志鸿,侯丫,郑言鑫..虾夷扇贝不同性别类型2个Dmrt基因DM结构域分析[J].海洋科学,2015,(3):19-25,7.基金项目
中国水产科学研究院黄海水产研究所基本科研业务费项目(20603022012003) （20603022012003）
山东省自主创新专项(2013CXC80202) （2013CXC80202）
