海洋科学Issue(3)：42-47,6.DOI:10.11759/hykx20140609001
响应面法优化香港巨牡蛎蛋白酶解工艺的研究
Improvement of Crassostrea hongkongensis hydrolysis by Response Surface Methodology
摘要
Abstract
Using DH (the degree of hydrolysis) as the maln index, the hydrolysis of Crassostrea hongkongensis using six proteases was compared. On the basis of single factor experiment, response surface methodology and Box-Behnken model were employed to establish the quadratic model for optimization of the hydrolysis conditions. Results showed that the DH of marine products protein-specific protease was higher than that of others. The opti-mum hydrolysis condition was as follows:the ratio of enzyme to substrate was 5.93%, hydrolysis time was 4.07 h and the hydrolysis temperature was 54.6℃. Under this condition, DH reached 25.73%±2.39%. This study alms to provide theoretical basis for the development and utilization of C. hongkongensis.关键词
香港巨牡蛎(Crassostrea hongkongensis)/水解度/响应面分析Key words
Crassostrea hongkongensis/Degress of hydrolysis/Response surface methodology (RSM)分类
轻工纺织引用本文复制引用
徐艳,孙雪萍,张秀国,杨家林,黄国强,张琴..响应面法优化香港巨牡蛎蛋白酶解工艺的研究[J].海洋科学,2015,(3):42-47,6.基金项目
广西科学院基本科研业务费资助项目(13YJ22HYS15) （13YJ22HYS15）
广西科学研究与技术开发计划项目(桂科攻09321004) （桂科攻09321004）
