渔业科学进展Issue(4)：22-31,10.DOI:10.11758/yykxjz.20140404
漠斑牙鲆（Paralichthys lethostigma）LHβ基因克隆及其在卵巢不同发育期的表达特征
Molecular Cloning and Differential Expression of LHβ Subunit During the Ovarian Maturation Cycle ofParalichthys lethostigma
摘要
Abstract
The role of the endocrine pathway in the regulation of southern flounder Paralichthys lethostigma reproduction has been unclear. Here we aimed to explore the underlying mechanisms by investigating the expression pattern of luteinizing hormoneβ subunit (LHβ). Using samples isolated from pituitary ofP. we obtained the full-length cDNA sequence of LHβ with the RACE method. The spatial and temporal expression patterns ofLHβmRNA in different tissues during an ovarian maturation cycle were analyzed using the qPCR assay. The levels of plasma LH and E2during an ovarian maturation cycle were also measured using the ELISA method. Our results revealed an asynchronous oocyte development and batch spawning in southern flounder. The cDNA sequence of LHβhad a length of 597 bp and it encoded 145 amino acids. LHβ expression was detected in all examined tissues except for muscle, which implied it could be involved in multiple physiological functions in southern flounder. The LHβ mRNA level in pituitary was high through the entire ovarian maturation cycle, and peaked at stage V and VI. In the ovary the LHβ mRNA level peaked at stage V. In the liver the LHβ mRNA level was the highest at stage II and relatively high at stage V. The levels of plasma LH and E2 fluctuated in a similar pattern during the ovarian maturation cycle. These results suggested that LHβmight regulate the ovarian maturation cycle via endocrine, paracrine and autocrine pathways. Our study provided insights into the functions of the endocrine pathway in the reproduction of southern flounder.关键词
漠斑牙鲆/LHβ亚基/卵巢发育周期/表达特性/血浆激素浓度Key words
Paralichthys lethostigma/LHβ subunit/Ovarian maturation cycle/Expression pattern/Plasma hormone levels分类
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柳学周,臧坤,徐永江,李春广,史宝,王妍妍..漠斑牙鲆（Paralichthys lethostigma）LHβ基因克隆及其在卵巢不同发育期的表达特征[J].渔业科学进展,2014,(4):22-31,10.基金项目
鲆鲽类现代化产业技术体系(CARS-50)和国家863计划项目(2012AA10A413)共同资助。 （CARS-50）
