渔业科学进展Issue(4)：71-76,6.DOI:10.11758/yykxjz.20140411
饥饿胁迫对刺参（Apostichopus japonicus）免疫和生长的影响
Starvation Stress Effect on the Immunity and Growth of Sea Cucumber Apostichopus japonicus
摘要
Abstract
In this study we investigated the effects of starvation on the growth and immunity of sea cucumberApostichopus japonicus.Sea cucumber seedlings with the initial weight of (20±0.15) g were exposed to starvation stress for different periods (0, 10, 20, 30, 40, 50, and 60 d). Non-specific immunity including activities of acidic phosphatase (ACP), lysozyme (LZM), superoxide dismutase (SOD), respiratory burst (RB), and phagocytic activity of coelomic fluid were examined to determine the effects of starvation on the immune responses. The body weight, the ratio of viscera to body wall (VBWR), and the survival rate were measured to determine the effects on the growth. The ACP and LZM activities decreased along with the starvation time: after a 60-day starvation the ACP and LZM activities decreased by 47.06% and 17.57% respectively. The activities of SOD, RB and phagocytosis first increased and then decreased during the 60-day starvation, and they reached their highest values on the 20th, 20th and 10th day respectively. On the 60th day the activities of SOD, RB, and phagocytosis were significantly lower and reached 27.87%, 38.08%, 53.43% of their initial values respectively. The body weights of the sea cucumbers constantly decreased along with the starvation time and reached 68.08% of the initial value on the 60th day. The VBWR increased gradually during the experiment and reached 0.56 on the 60th day which was significantly higher than the initial value 0.44 (P<0.05). The loss rate of the body weight increased and the survival rate decreased along with the starvation time. There was a negative quadric linear correlation between the loss rate of the body weight and the survival rate.These results indicated that sea cucumbers could consume the nutrients in the body wall to meet their needs of energy during starvation. Long-term starvation might cause suppression of immune activities thus affect the health and growth of sea cucumbers.关键词
刺参/饥饿胁迫/免疫/负生长/存活率/脏壁比Key words
Sea cucumber/Starvation stress/Immunity/Negative growth/Survival rate/Ratio of viscera to body wall分类
农业科技引用本文复制引用
田青,荣小军,李彬,廖梅杰,姜燕,范瑞用,王印庚,李强..饥饿胁迫对刺参（Apostichopus japonicus）免疫和生长的影响[J].渔业科学进展,2014,(4):71-76,6.基金项目
国家863计划(2012AA10A412-4)、山东省自主创新成果转化专项(2013ZHZX2A0801)和青岛市战略性新兴产业培育计划项目(13-4-1-65-hy)共同资助。 （2012AA10A412-4）
