渔业科学进展Issue(4)：91-96,6.DOI:10.11758/yykxjz.20140414
Pb2+和Cd2+对刺参幼参的急性毒性及其富集
Acute Toxicity and Accumulation of Pb2+ and Cd2+ in Juvenile Sea Cucumber Apostichopus japonicus
摘要
Abstract
To improve the quality of the aquaculture of sea cucumberApostichopus japonicus and to enhance the environmental restoration, we studied the acute toxicity of Pb2+ and Cd2+ on sea cucumber and the bio-accumulation of these two heavy metals. Using static bio-assay we divided the experimental subjects into groups exposed to each heavy metal at a different concentration-- Pb2+ (0.1, 0.25, 0.5, 1.0, 2.5 and 5.0 mg/L), and Cd2+ (0.2, 0.4, 0.8, 1.0, 2.5 and 5.0 mg/L), as well as the blank control. We found that mortality rate ofA. japonicus increased along with the exposing period or ion concentration, and the adhesion rate showed an opposite pattern. There was no significant difference in the mortality rates between the two groups exposed to low concentrations of Pb2+ (P > 0.05), but the mortality rates of other groups rose along with the increase in exposing periods or Pb2+concentration. When exposed to 5.0 mg/L of Pb2+, the mortality rate reached 100% in 72 h and the adhesion rate decreased to 6.7% in 24 h, which were significantly different from other Pb2+ groups (P< 0.01). When exposed to Cd2+, the mortality rate ofA. japonicustreated for 72 h was apparently higher than that after the 48 h treatment; in 96 h the mortality rate reached 100% in the group treated with 0.8 mg/L Cd2+. Generally the adhesion rate ofA. japonicus was lower after the Cd2+ treatment. The safe concentrations of Pb2+ and Cd2+ were 0.061 mg/L and 0.018 mg/L respectively. The concentrations of the heavy metals and their accumulation rates inA. japonicus were elevated along with the increase in concentrations of Pb2+ and Cd2+ in the water, with a remarkable fluctuation in the concentration factor. Both the concentration factor and the accumulation rate of Cd2+ in juvenilesea cucumber were much higher than those of Pb2+. Therefore, the acute toxicity and the accumulation of Cd2+ may be higher than those of Pb2+. This study should provide insights into the ecological restoration ofA. japonicus, and scientific references for the aquaculture quality and seafood safety.关键词
Pb2+/Cd2+/刺参/急性毒性/富集Key words
Pb2+/Cd2+/Apostichopus japonicus/Acute toxicity/Accumulation分类
农业科技引用本文复制引用
邢红艳,任利华,马元庆,李斌,刘爱英,靳洋,孙珊,白艳艳,赵强,苏博..Pb2+和Cd2+对刺参幼参的急性毒性及其富集[J].渔业科学进展,2014,(4):91-96,6.基金项目
烟台市科技发展计划项目(2011457)、国家自然科学基金项目(41206094)和“水生动物营养与饲料泰山学者岗位”课题共同资助。 （2011457）
