渔业科学进展Issue(4)：103-109,7.DOI:10.11758/yykxjz.20140416
一株海洋过氧化氢酶高产菌的鉴定及产酶条件优化
Identification of a Marine Bacterium Producing High-Level Catalase and Optimization of Its Fermentation Conditions
摘要
Abstract
Catalases are a type of enzymes that can effectively decompose hydrogen peroxide into water and oxygen. Because of their ubiquitous distribution in all aerobic microorganisms, plants and animals, they are widely used in food, pharmaceutical, chemical industries and environmental protection. In this study we examined the bacterial strain YS0810 collected from the sediment in Yellow Sea that produces catalase at a high level. We determined the growth conditions of this strain which is optimal for the catalase production. Phylogenetic analysis of the 16S rDNA of this strain was applied to determine its taxonomic rank. The conventional morphological, physiological and biochemical methods of taxonomy were also applied to differentiate the YS0810 from its phylogenetic relatives. We used 250 ml shake flasks to carry out the single factor experiments to identify the important growth factors for the strain YS0810. The phylogenetic tree indicated that strain YS0810 belonged to the genusAcinetobacter, with the highest sequence similarity toAcinetobacter haemolyticus DSM 6962T (98.7%). However, YS0810 was not a strain ofA. haemolyticus. This was consistent with the results of the comparison between YS0810 and otherAcinetobacterspecies in terms of their morphological, physiological and biochemical characteristics. Peptone and sucrose were determined as the optimal nitrogen and carbon sources from several candidates. Our experimental results indicated that the maximum yield of the catalase was generated by YS0810 under the conditions shown below: peptone 15 g/L, sucrose 20 g/L, MgSO4·7H2O 0.9 g/L, NaCl 5.0 g/L, KH2PO4 1.0 g/L, broth content at 50 ml, the inoculum at 4%, initial pH 7.0, the temperature at 25°C and the culture time of 24 h. The verification experiment carried out in the optimal conditions above showed that the highest catalase activity was 2469 U/ml, which is five-time higher than before the optimization. In conclusion, the yield of catalase was markedly raised in the optimized fermentation conditions.关键词
海洋微生物/过氧化氢酶/不动杆菌属/鉴定/培养条件优化Key words
Marine microorganism/Catalase/Acinetobacter/Identification/Culture optimization分类
农业科技引用本文复制引用
陈世建,王伟,孙谧..一株海洋过氧化氢酶高产菌的鉴定及产酶条件优化[J].渔业科学进展,2014,(4):103-109,7.基金项目
国家“十二五”863项目(2011AA090703)和国家自然科学基金(41006119)共同资助。 （2011AA090703）
