渔业科学进展Issue(6)：23-29,7.DOI:10.11758/yykxjz.20140604
青岛陆源排污口邻近海域异养细菌的组成与分布
Diversity and Distribution of Marine Heterotrophic Bacteria Nearby Qingdao Land-Based Drainage Discharge Outlets
摘要
Abstract
Three hundred and twelve strains of heterotrophic bacteria were isolated from the seawater of Qingdao land-based drainage discharge outlets. The sampling locations included tourist area, port area, aquaculture area and industrially affected region. Based on the analysis of 16S rDNA and Amplified Ribosomal DNA Restriction Analysis(ARDRA), these strains were clustered and identified. Four phyla including Proteobacteria, Firmicutes, Actinobacteria and Bacteroidetes were identified. Sixty-eight percent of strains belonged to Proteobacteria, and 88% of Proteobacteria were classified into Gammap-roteobacteria. The diversity and distribution of the bacteria among each region were different, and a few of them were Actinobacteria and Bacteroidetes. Vibrio sp. was found in all four regions, and it accounted for more than 18% of strains. Twenty-five percent of Vibrio sp. was V. alginolyticus. Many pathogenic bacteria such as V. parahaemolyticus, V. harveyi, V. campbellii, and V. parahaemolyticus were found in all samples. Based on analysis of 16S rDNA and clustering, the diversity and distributions of bacteria between industrially affected region and aquaculture area were highly similar. These results suggest a close relationship between the environment and the diversity and distribution of bacteria among different regions.关键词
排污口/海洋异养细菌/16S rDNA/弧菌Key words
Discharge outlet/Marine heterotrophic bacteria/16S rDNA/Vibrio sp分类
农业科技引用本文复制引用
刘霜,李永霞,刘旭东,崔正国,王亚楠,孙蓓蓓,宋帅..青岛陆源排污口邻近海域异养细菌的组成与分布[J].渔业科学进展,2014,(6):23-29,7.基金项目
海洋公益性行业科研专项(201105007)资助 （201105007）
