渔业科学进展Issue(6)：68-75,8.DOI:10.11758/yykxjz.20140610
盐度对寄主暗纹东方鲀（Takifugu obscurus）存活影响及其对车轮虫的杀灭作用
The Effects of Salinity on the Survival of Takifugu obscurus and Annihilation of Its Parasite, Trichodina sp.
摘要
Abstract
Trichodiniasis is a common fish disease and has recently become a serious issue in fugu fish culture in China. In this study we investigated the effects of salinity on the survival of fugu, Takifugu obscurus, and annihilation of the parasites, Trichodina sp., on the fish body surface. We set up 13 salinity gradient groups (0, 2.5, 5.0, 7.5, 10.0, 12.5, 15.0, 17.5, 20.0, 25.0, 30.0, 35.0, and 40.0) for the experiment. The results showed that T. obscurus could survive safely under salinity 15.0. At low salinity (0, 2.5 or 5.0) Trichodina on the body surface was not reduced, and tended to increase in 4 days. However, more than 95%of Trichodina could be killed at the salinity 7.5 for 4 days, 10.0 for 3.5 days, or 12.5 for 3 days. The parasites could be completely killed if treated at salinity 15.0 for 32 h, 17.5 for 24 h, 20.0 for 10 h, 25.0 for 4 h, 30.0 for 1.5 h, 35.0 for 1 h, or 40.0 for 0.5 h. A fitting curve equation of salinity and the killing time for Trichodina was established using the software SPSS, and the logarithmic curve equation was y=34.19-5.55lnx. Moreover, according to the resurgence test of Trichodina sp. on fish body surface, Trichodiniasis in T. obscurus did not reoccur when the salinity returned back to 5.0 during farming if Trichodina sp. were entirely killed by the raised salinity. Our results suggested that trichodiniasis in T. obscurus could be cured if salinity was maintained higher than 7.5. Salinity between 7.5 and 15.0 could be proper for the treatment of the trichodiniasis in T. obscurus. Our study should provide insights and technical supports for clinical treatment of trichodiniasis in fugu farming.关键词
暗纹东方鲀/盐度/车轮虫/灭杀/存活Key words
Takifugu obscurus/Salinity/Trichodina/Kill/Survival分类
农业科技引用本文复制引用
刘金海,张正,王印庚,郭正龙,武宁宁..盐度对寄主暗纹东方鲀（Takifugu obscurus）存活影响及其对车轮虫的杀灭作用[J].渔业科学进展,2014,(6):68-75,8.基金项目
国家科技支撑计划课题“海洋水产病害综合防控技术的集成与示范”(2012BAD17B03)资助。 （2012BAD17B03）
