渔业科学进展Issue(6)：97-102,6.DOI:10.11758/yykxjz.20140614
马氏珠母贝转录组数据的重新组装
Data Reassembling Reveals More Information of Transcriptome for Pinctada fucata martensii Dunker
摘要
Abstract
The pearl oyster, Pinctada fucata martensii, was an ideal animal for studies of biomineralization. The mantle and pearl sac were the two important tissues involved in biomineralization of the shell and pearls. In this study we reassembled the transcriptome data of 454 pyrosequencing of the mantle and pearl sac. The sources of the data included studies in our own laboratory, reports from other researchers, and ESTs from NCBI. We conducted BLAST and Gene Ontology analysis, and analyzed the contig reassembling with CLC Genomics Workbench 5. The reassembling generated 30266 contigs with an average length of 522 bp, and the largest length of 4144 bp. The Blast analysis against non-redundant (NR) database revealed 2310 contigs with the E-values smaller than 10-5, among which 1902 contigs had homologous sequences in at least one of the following model animals of Homo sapiens, Mus musculus, Strongylocentrotus purpuratus, Caenorhabditis elegans, Danio rerio, Drosophila melanogaster, or Crassostrea gigas and Pinctada. The GO annotation showed that 3846 contigs could be characterized into 23 subcategories of biological processes, of which the top three subcategories with most contigs were metabolic process, cellular process and biological regulation;4601 contigs could be characterized into 11 subcategories of molecular functions, of which the top three with most contigs were binding, catalytic activity, and structural molecular activity; 2992 contigs could be characterized into 17 subcategories of cellular components, of which the top three were cell part, organelle, and macromolecular complex. These results suggested that there were a great number of transcriptome contigs of P. fucata martensii. Our study will be helpful in the large-scale screening of specially-expressed genes in the mantle and pearl sac of P. fucata martensii.关键词
马氏珠母贝/外套膜/珍珠囊/转录组/454焦磷酸测序Key words
Pinctada fucata martensii/Mantle/Pearl sac/Transcriptom/454 Pyrosequencing分类
农业科技引用本文复制引用
陈琼,顾志峰,王爱民,战欣,石耀华..马氏珠母贝转录组数据的重新组装[J].渔业科学进展,2014,(6):97-102,6.基金项目
国家自然科学基金(41366003)和国家重点基础研究发展计划(973计划)(2010CB126405)共同资助。 （41366003）
