渔业科学进展Issue(6)：103-109,7.DOI:10.11758/yykxjz.20140615
基于遗传连锁图谱筛选虾夷扇贝性别相关AFLP分子标记的方法
Screening the Sex-Related AFLP Molecular Markers in Patinopecten yessoensis Based on the Genetic Linkage Map
摘要
Abstract
Patinopecten yessoensis is one of the most important germplasm resources in scallop breeding in north coast of China. It has male, female and hermaphrodite phenotypes. A rising hermaphroditism percentage in cultured populations was observed in gonochorism P. yessoensis. The previous studies indicated that hermaphroditisms were intermediate states of sex-reversal, and that the development of gonad was simultaneous in some hermaphroditic scallops. The hemaphroditisms could provide useful materials for inbred line construction and genetic improvement of scallop. AFLP (amplified fragment length polymorphism) markers have been used to construct the genetic linkage maps in the scallop P. yessoensis by several research groups since 2009. The sex-related AFLP molecular markers were utilized to screen female, male and hermaphrodite P. yessoensis from samples that were collected from March to May, 2011–2013. Eighteen AFLP primer-combinations were chosen to screen sex-related bands and 8 primer-combinations were sex-specific markers. Three of eight markers (EbMc, EjMf and EiMk) had a good repeatability. Hermaphrodite-specific bands were found in four primer-combinations and female-specific bands were detected in five primer-combinations. The present results indicate that the method of sex-related molecular markers screening based on the highly dense genetic linkage map was practical and feasible, and that the hermaphrodism is probably an independent group since the sex-related molecular markers in hermaphrodite genomic DNA were apparently different from those of the female and male. The method of sex-related markers screening and the sex-related bands screened may contribute to the efficiency improvement of sex control and breeding, and help to identify the hermaphrodite and then induce the female-and male-gametes to mature synchronously to construct self-bred-lines.关键词
虾夷扇贝/性别相关标记/AFLP/雌雄同体Key words
Patinopecten yessoensis/Sex-related markers/AFLP/Hermaphrodite分类
农业科技引用本文复制引用
周丽青,杨爱国,刘志鸿,王清印,吴彪,孙秀俊,郑利兵,郑言鑫..基于遗传连锁图谱筛选虾夷扇贝性别相关AFLP分子标记的方法[J].渔业科学进展,2014,(6):103-109,7.基金项目
黄海所基本科研业务费项目(20603022012003)和国家科技基础条件平台项目(2007DKA30470)共同资助。 （20603022012003）
