渔业科学进展Issue(1)：41-47,7.DOI:10.11758/yykxjz.20150106
牙鲆(Paralichthys olivaceus) Activin 基因两种β亚基的启动子克隆及生物信息学分析
Bioinformatic Characterization of Promoters of Two Activin-βSubunit Genes in Paralichthys olivaceus
摘要
Abstract
Activin is a member of the transforming growth factor-β (TGF-β) family and regulates sex hormones. It was originally discovered in pig ovarian follicular fluid. Activin contains two β subunits and plays a vital role in the hypothalamus-pituitary-gonad axis (HPG). It regulates the secretion of pituitary gonadotropin, the production of steroid hormones and the maturation of oocyte in ovary. Paralichthys olivaceus is a type of important commercial fish species that has advantageous traits in aquaculture such as the fast growth rate. Better understanding of its reproduction mechanism is essential for the guidance of the breeding of P. olivaceus. In this study, we analyzed the expression and regulation of Activin gene related to the reproductive endocrinology of P. olivaceus. Our data should provide important information for future studies on biological functions of Activin and for the practice in the culture of P. olivaceus. We used the genome walking method to obtain the partial sequence of promoters located in the upstream of Activin βA and βB genes of P. olivaceus, and predicted the binding sites of transcriptional regulation elements using the bioinformatical method. The promoters of these two genes were 2.7 kb and 2.4 kb in length respectively. The results showed that the TATA box of Activin βB was located at 31 bp in the upstream region of the transcription initiation site, however this structure was not found in Activin βA. We found in the two promoters a number of binding sites of the transcription factors including Sp1, Oct-1, C/EBP, CREB, GATA-1, c-Jun, HNF-3, HNF-1, and USF. Moreover, we also found multiple transcription binding sites of endocrine-related factors such as Pit-1, ER, PR, GR, RAR, and RXR. However, the binding sites of MyoD, myogenin and SRY were only found in Activin βA. In conclusion, the bioinformatical analysis suggested that the basic and hormone-inducing expression of both Activin βA and βB was regulated by a variety of factors; however, the mechanisms of the regulation were different between the two genes.关键词
牙鲆/激活素/启动子/生物信息学分析Key words
Paralichthys olivaceus/Activin/Gene promoter/Bioinformatics analysis分类
农业科技引用本文复制引用
刘蒙蒙,王晶,高金宁,马丽曼,张全启..牙鲆(Paralichthys olivaceus) Activin 基因两种β亚基的启动子克隆及生物信息学分析[J].渔业科学进展,2015,(1):41-47,7.基金项目
国家863计划课题(2012AA10A402 （）
2012AA10A408)资助。 （）
