渔业科学进展2015，Vol.36Issue(2)：45-54,10.DOI:10.11758/yykxjz.20150206
半滑舌鳎(Cynoglossus semilaevis)体表色素细胞观察及POMC表达特性分析
Preliminary Studies on Blind-side Hypermelanosis ofCynoglossus semilaevis: Chromatophores Observation and Expression of Proopiomelanocortin
摘要
Abstract
The hypermelanosis on the blind-side ofCynoglossus semilaevis have reduced its market value and affected its availability in the enhancement program, which has adverse effects on the sustainable development of the farming industry. However the underlying mechanisms of hypermelanosis have not been clarified yet. To investigate the cytological mechanism of hypermelanosis on the blind-side ofC. semilaevis, we identified three kinds of chromatophores, namely melanophore, xanthophore and iridophore, based on the microscopic observation, and we described their morphology. The melanophore contained black and brown pigment granules and had a relatively larger nucleus. They could be characterized into two types according to the morphology. In contrast, the nuclei of xanthophore and iridophore were relatively small. The distribution and pattern of the three chromatophores in the eye-side and the blind-side skin were examined and compared. We isolated and sequenced the proopiomelanocortin (POMC) cDNA from pituitary using RT-PCR and RACE. The POMC cDNA was 910 bp in length consisting of a 114 bp 5'-UTR, a 154 bp 3'-UTR, and a 642 bp open reading frame that encoded 213 aminoacids. The amino acid sequence was composed of the signal peptide, ACTH,α-MSH,βMSH,γ-LPH, andβ-endorphin domains, withoutγ-MSH and most of the joining peptide domains. The amino acid homology analysis revealed thatC. semilaevis POMC shared 30%?64% similarity with their fish counterparts. Quantitative PCR results showed that POMC mRNA was mainly expressed in the pituitary and was also expressed in the brain, gonad and the black skin (hypermelanosis) on the blind side. The POMC mRNA expression level in the blind-side black skin was significantly higher than that in the ocular-side and in the blind-side normal skin, which indicated a close correlation between the POMC gene and the hyperpigmentation on the blind-side. Our study provided basic information on the cytological and molecular mechanisms of hyperpigmentation on the blind-side ofC. semilaevis.关键词
半滑舌鳎/色素细胞/POMC/基因克隆/组织表达Key words
Cynoglossus semilaevis/Chromatophores/Proopiomelanocortin/Molecular cloning/Spatial expression分类
农业科技引用本文复制引用
史学营,徐永江,武宁宁,柳学周,杨洪军,臧坤,史宝,李存玉..半滑舌鳎(Cynoglossus semilaevis)体表色素细胞观察及POMC表达特性分析[J].渔业科学进展,2015,36(2):45-54,10.基金项目
国家鲆鲽类产业技术体系(CARS-50)、国家国际科技合作专项项目"近海衰退渔业种群重建关键技术合作研究"(2013DFA31410)和国家留学人员科技活动项目择优资助经费共同资助 （CARS-50）
