渔业科学进展2015，Vol.36Issue(2)：55-62,8.DOI:10.11758/yykxjz.20150207
利用微卫星标记分析3个暗纹东方鲀 (Takifugu obscurus)养殖群体的遗传多样性
Study on the Genetic Structure of Three Cultured Populations of Takifugu obscurus with Microsatellite Markers
摘要
Abstract
In recent years the aquacultureTakifugu obscurus has been impeded due to the adverse changes in the ecological environments and in the hydrological conditions of the Yangtze River. Here we evaluated the genetic diversity of three cultured populations ofT. obscurusandhence provided theoretical data for the potential improvement inT. obscuresgenetics. We used 21 pairs of microsatellite primers to analyze the genetic variances between the populations with SSR molecular marker technique, and successfully amplified19 microsatellite markers. In three populations ofT. obscurus,the number of alleles (A) was 3?11 with an average of 6.58; the effective number of alleles (Ne) was 1.7?7.8 with an average of 4.5; the value of average observed heterozygosities was 0.156?1.000; the expected heterozygosities (He) was 0.399?0.876 and the mean polymorphic information content (PIC) ranged from 0.353 to 0.858. The mean polymorphic information content (PIC) followed the order of Guangzhou population (0.588) ＜ Shanghai population (0.633) ＜ Jiangsu population (0.655). The genetic diversity coefficients were 0.048, 0.062, and 0.076 respectively with an average of 0.081. There was moderate genetic differentiation among the three populations. The genetic distances between each population and the UPGMA dendrogram showed that Guangzhou population and Shanghai population had the most remote relationship (0.351), and that Guangzhou population and Jiangsu population were the closest (0.204). The three populations were pooled into 2 groups in the cluster analysis—the Guangzhou/Jiangsu group and the Shanghai group. These results suggested that there was a low level of polymorphism information content and moderate genetic diversity in the three populations ofT. obscurus. Noticeably the genetic diversity of the farmed Jiangsu population was higher than that of the farmed Guangzhou and Shanghai populations, so there was a high potential in selective breeding. Our study broadened the knowledge on the genetics ofT. obscurus and provided necessary information for the artificial breeding.关键词
暗纹东方鲀/养殖群体/微卫星/遗传多样性Key words
Takifugu obscurus/Cultured population/Microsatellite/Genetic diversity分类
生物科学引用本文复制引用
邹杰,马爱军,赵艳飞,何伟国,王婷,岳亮,刘大勇,郭正龙..利用微卫星标记分析3个暗纹东方鲀 (Takifugu obscurus)养殖群体的遗传多样性[J].渔业科学进展,2015,36(2):55-62,8.基金项目
江苏省科技支撑计划(BE2013345)和江苏省水产三新工程项目(Y2013-12)共同资助 （BE2013345）
