杭州师范大学学报（自然科学版）Issue(3)：263-268,6.DOI:10.3969/j.issn.1674-232X.2015.03.007
卵源性多价抗蝮蛇毒IgY的制备及检测
Preparation and Detection of Polyvalent IgY Antibodies against Pit Vipers
摘要
Abstract
The experiment uses the toxoid prepared from the complexity of snake venoms from Gloydius brevicaudus , Gloydius ussuriensis ,Gloydius saxatilis ,Protobothrops mucrosquamatus and Protobothrops jerdonii to immunize the 20~22 week‐old Leghom hens ,and collects the eggs before and during the immunization ,then cracks the eggs and separates the yolk to extract the polyvalent egg yolk IgY by freeze‐thawing method ,water dilution method and PEG extraction method , and determines the titer of IgY by ELISA and Western blot . The results show all of the eggs collected during the immunization contain effective IgY that can neutralize five snake venoms mentioned above ,and the content of these IgY increases as the immune‐intensity enhancing .The content of effective IgY varies by different extraction methods ,and even the efficiency of IgY extracts by the same method varies while neutralizes with different venoms ,this might be attributed to the different immune response of animals to these five venoms .关键词
卵黄/多价Ig Y/蝮蛇/蛇毒/酶联免疫法/蛋白印迹法Key words
egg yolk/polyvalent IgY/pit viper/snake venom/ELISA/Western blot分类
生物科学引用本文复制引用
单琳琳,陈传稀,蔡梦思,黄利锋,何莹,高建芳..卵源性多价抗蝮蛇毒IgY的制备及检测[J].杭州师范大学学报（自然科学版）,2015,(3):263-268,6.基金项目
浙江省自然科学基金项目（LY14C030007）；浙江省大学生科技创新活动暨新苗人才计划项目（2013R421051）；杭州师范大学本科生创新能力提升工程项目（CX2014097）． （）
