家畜生态学报Issue(9)：14-17,4.
Lin28B基因表达与绵羊初情期启动的关系研究
Lin28B Gene Expression and its Role in the Onset of Puberty of Sheep
摘要
Abstract
Puberty of animals marked their fertility and is a key stage in female animal development . Precocious sheep breed ,Duolang sheep ,and late-maturing sheep breed ,Hetian sheep were selected as sub-jects in the study ,the exon 3 of Lin28B gene of the selected animals were cloned ,and the real-time PCR technology was used to examine the mRNA expression level of Lin28B gene in the hypothalamus and ovary of juvenile stage and pubertal stage .The results showed the sequences alignment of Lin28B exons 3 of Duo-lang sheep and Hetian sheep were the same ;the expression level of Lin28B gene in the hypothalamus and ovary were significantly lower than that in juvenlie period of Duolang sheep (P< 0 .05) ,the expression lev-el of Lin28B gene in the ovary was significantly lower than that in the juvenlie period of Hetian sheep (P<0 .05) .The results indicated that the down-regulated expression of Lin28B gene was positively correlated with the onset of puberty in sheep .The study can help to provide a scientific basis for revealing the effect and mechanism of Lin28B gene in the onset of puberty in sheep .关键词
多浪羊/Lin28B/初情期/荧光定量PCRKey words
Duolang sheep/Lin28B/puberty/qPCR分类
农业科技引用本文复制引用
卢萍平,邢凤,刘博丹,梁志鹏..Lin28B基因表达与绵羊初情期启动的关系研究[J].家畜生态学报,2014,(9):14-17,4.基金项目
塔里木大学校长基金项目（TDZKBS20103）；国家大学生创新创业训练计划项目 （）
