极地研究Issue(2)：212-221,10.DOI:10.13679/j.jdyj.2014.2.212
基于mtCO I片段序列的南极海域浮游动物DNA条形码研究
DNA BARCODING OF ANTARCTIC MARINE ZOOPLANKTON FOR SPECIES IDENTIFICATION AND RECOGNITION
摘要
Abstract
Polar zooplankton are particularly sensitive to climate change,and have been applied as‘rapid-responders’of climate-induced change in this fragile ecosystem.DNA barcoding provides an alternative approach for rapid species identification of zooplankton in the Southern Ocean,which will accelerate the routine monitoring of zooplankton community structure.Ninety-four specimens belonging to 32 Antarctic zooplankton were barcoded to provide a more comprehensive reference library.An 830 to 1 050 base-pair region of the mitochondrial cytochrome c oxidase subunit I (mtCOI)gene was obtained as a DNA barcode.The intraspecific variation of the gene ranged from 0 to 2.6%(p-distance),with an average of 0.67%(SD=0.67%).The counterpart between species within the same genera ranged from 0.1% (Calanus)to 29.3%,with an average of 1 5.3% (SD =8.4%).The morphological and genetic similarities between C.propinquus and C.simillimus raise new questions about the status of C.simillimus as a different species.With the exception of the two Calanus species (C.propinquus and C.simillimus ),the intraspecific genetic divergence was much smaller than interspecific divergence among the congenus species, confirming the existence of a barcode gap for Antarctic zooplankton.In addition,all species clustered into a mono-phyletic clade except for Calanus species.Hence,DNA barcoding is confirmed as an accurate and efficient approach for zooplankton identification in the Southern Ocean (species belonging to jellyfish and doliolum were not tested).Confirming evidence was also provided by indicator vector analysis.The new primer sets issued here will facilitate study of the species composition of Antarctic marine zooplankton by single-gene based environmental metagenomic analysis.关键词
南大洋/DNA条形码/哲水蚤属/浮游动物Key words
Southern Ocean/DNA barcode/Calanus/zooplankton引用本文复制引用
程方平,王敏晓,孙松,李超伦,张永山..基于mtCO I片段序列的南极海域浮游动物DNA条形码研究[J].极地研究,2014,(2):212-221,10.基金项目
中国极地科学战略研究基金项目（20100215，20120308）、南北极环境综合考察与评估专项（CHINARE2013-01-05）和中国科学院海洋所海洋生物多样性专项资助 （）
