激光生物学报Issue(3)：218-226,9.DOI:10.3969/j.issn.1007-7146.2014.03.006
菟丝子总黄酮对内分泌衰退痴呆模型小鼠学习记忆功能的影响及保护作用机制
The Protective Mechanism of Total Flavonoids from Semen Cuscutae(TFSC)on Learning-memory Function in Alzheimer’s Disease Mice with Hypoendocrinism
摘要
Abstract
Objective:To discover and research the influence and mechanism of total flavonoids from semen cuscutae (TFSC)on learning-memory function of Alzheimer’s disease mice with hypoendocrinism.Methods:50 female Kunming mice were randomly divided into five groups:model group,control group with estrogen (0.3 mg·kg-1 ),experimental groups with low-dose(35 mg·kg-1 ),middle-dose(70 mg·kg-1 ),high-dose(140 mg·kg-1 )of TFSC treatment.The mod-el of Alzheimer disease with hypoendocrinism were built by removing bilateral ovaries from backside .Another 10 female Kunming mice were treated with sham operation as the control group.After ovariectomization,four groups of mice were intragastric administrated with estrogen or TFSC for three months,sham operation group and model group mice were in-tragastric administrated with NS.The learning-memory ability of the mice were assessed by Morris water maze behavioral test;The levels of estrogen in serum were tested by radioimmunoassay;Neuronal pathological morphology in hippocam-pus were observed through hematoxylin-eosin staining(HE).The apoptotic rate of hippocampal neurons were tested by flow cytometry;The expression of apoptosis-related proteins (Bcl-2,Bax,Caspase-3)and Cyt-c in hippocampal neu-rons were measured by Western blotting.Results:Compared with sham operation group,the abilities of learning-memory of the mice in model group were apparently declined(P<0.01);The estrogen level of serum in model mice significantly reduced(P<0.01);The apoptosis rate of hippocampal neurons in model mice significantly increased(P<0.01);the expression of Bax,Caspase-3 and Cyt-c of hippocampal neuron of model mice significantly reduced(P<0.01),the ex-pression of Bcl-2 increased significantly(P<0.01).Compared with the model group,the abilities of learning-memory of the mice of TFSC treatment significantly increased(P<0.05,P<0.01,P<0.01),the estrogen level of serum rised(P<0.05,P<0.01,P<0.01);Apoptotic rate and expression of Caspase-3,Bax,Cyt-c detected from hippocampal neu-rons remarkably decreased.In contrast,the level of Bcl-2 increased(P <0.05,P<0.05,P<0.01).Conclusions:TF-SC can improve the expression of Bcl-2 and inhibit the proteins of Bax,Caspase-3 and Cyt-c in hippocampal neurons to block the apoptosis from endogenously mitochondria apoptosis pathway.TFSC can also enhance the plasticity and nutri-tion of neurons in order to improve the learning-memory ability of Alzheimer’s disease mice with hypoendocrinism.TFSC may improve the function of hypothalamus-pituitary-gonad axis to regulate estrogen levels and improve endocrine system.关键词
菟丝子总黄酮/内分泌衰退痴呆模型/雌激素/学习记忆/细胞凋亡Key words
Total flavonoids from semen cuscutae (TFSC)/model of Alzheimer disease with hypoendocrinism/es-trogen/learning and memory/apoptosis分类
医药卫生引用本文复制引用
彭申明,陈勤,陈逸青,叶海燕,王亭..菟丝子总黄酮对内分泌衰退痴呆模型小鼠学习记忆功能的影响及保护作用机制[J].激光生物学报,2014,(3):218-226,9.基金项目
安徽省教育厅自然科学研究项目（KJ2012A031）；《细胞生物学》省级精品课程项目（2009024）；安徽省教育厅教学研究项目（2012jyxm080）；教育部大学生创新性实验项目 （）
