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法夫酵母 JMU-MVP14菌体中类胡萝卜素成分分析

李慧翔 伍菱 黄高凌 杨远帆 肖安风 杜希萍

激光生物学报Issue(3):234-238,263,6.
激光生物学报Issue(3):234-238,263,6.DOI:10.3969/j.issn.1007-7146.2014.03.008

法夫酵母 JMU-MVP14菌体中类胡萝卜素成分分析

Analysis of Carotenoids in Phaffia rhodozyma JMU-MVP1 4

李慧翔 1伍菱 1黄高凌 2杨远帆 3肖安风 4杜希萍1

作者信息

  • 1. 集美大学生物工程学院,福建 厦门361021
  • 2. 福建省食品微生物与酶工程重点实验室,福建 厦门361021
  • 3. 福建省高校食品微生物与酶工程技术研究中心,福建 厦门361021
  • 4. 厦门市食品与生物工程技术研究中心,福建 厦门361021
  • 折叠

摘要

Abstract

Carotenoids in Phaffia rhodozyma JMU-MVP14 were analyzed by combining the methods of TLC, CLC (column liquid chromatography)and HPLC.With the combining of MgO-CLC and silica gel-CLC,carotenoids in Phaffia rhodozyma JMU-MVP14 were separated well into several fractions,each fraction with an individual carotenoid. Unsatisfactory separation and drifting of Rt (retention time)in HPLC method in which ODS column was employed,and shortage of available commercial carotenoid standards made it difficult to identify the carotenoids in Phaffia rhodozyma JMU-MVP14.The method mentioned in this paper overcame the above defects.

关键词

法夫酵母/类胡萝卜素/柱层析/HPLC

Key words

Phaffia rhodozyma/carotenoids/CLC/HPLC

分类

生物科学

引用本文复制引用

李慧翔,伍菱,黄高凌,杨远帆,肖安风,杜希萍..法夫酵母 JMU-MVP14菌体中类胡萝卜素成分分析[J].激光生物学报,2014,(3):234-238,263,6.

基金项目

福建省自然科学基金项目(2012J05058);李尚大集美大学学科建设基金(ZC2012006);福建省发改委产业技术开发专项 ()

激光生物学报

OACSTPCD

1007-7146

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