激光生物学报Issue(3)：270-274,5.DOI:10.3969/j.issn.1007-7146.2014.03.014
生物传感器法测定麦芽汁中赖氨酸含量的研究
A Study of Determination for Lysine in Wort by Biosensor
冯东 1王丙莲2
作者信息
- 1. 山东省科学院生物研究所，山东 济南250014
- 2. 山东省生物传感器重点实验室，山东 济南250014
- 折叠
摘要
Abstract
Based on immobilized lysine oxidase and biosensor,method for the determination of lysine content in wort was established.The liner range,detection limit,optimum pH,stability and specificity were studied.Measurement of wort was compared with other determination ways such as amino acid analyzer,DBL,HPLC.The results showed that this method was very simple and accurate.The recovery ratio was 97.41%-103.23%,and relative standard deviation (RSD)2.23%.This method had a good linear relationship within the scope of 2~100 mg/100mL.And the detection limit was 2 mg/100 mL.This determination method for lysine had a series of characteristics such as high specificity and high precision,suitable for determination of lysine content in wort with a variety of amino acids.There were no signifi-cant differences (P>0.05)in measurement results among biosensor,DBL and HPLC.关键词
麦汁/啤酒生产/赖氨酸/生物传感器Key words
wort/beer production/lysine/biosensor分类
信息技术与安全科学引用本文复制引用
冯东,王丙莲..生物传感器法测定麦芽汁中赖氨酸含量的研究[J].激光生物学报,2014,(3):270-274,5.
