激光生物学报Issue(3)：283-288,6.DOI:10.3969/j.issn.1007-7146.2014.03.017
基于 LUX 引物的 HBV 实时 PCR检测方法的建立
Development of a Real-time PCR Method for HBV Detection Based on LUX Primer
摘要
Abstract
Real-time PCR technology has been widely used in molecular biology and medical research due to its im-proved rapidity,accuracy,sensitivity,reproducibility and reduced risk of carry-over contamination.A quantitative real-time PCR method based on Light Upon Extension (LUX)primer was established to measure the viral load of hepatitis B virus in serum.The performance of the LUX real-time assay was validated by testing serial dilutions of HBV DNA (5 to 5 ×108 copies per reaction)and a good linear relationship was obtained between the Ct values and the log10 concentration of the HBV DNA.The LUX method possessed high sensitivity and the detection limit of this system was as few as 25 copies/mL of serum.9 1 positive serum samples were detected to further evaluate the assay and the high specificity was confirmed by melting curve analysis.This assay provides an ideal approach for monitoring the treatment efficacy and studying the relationship between HBV viral load and stage of disease.关键词
乙型肝炎病毒/LUX/实时PCR/定量Key words
Hepatitis B virus/light upon extension/real-time polymerase chain reaction/quantification分类
生物科学引用本文复制引用
李桂民..基于 LUX 引物的 HBV 实时 PCR检测方法的建立[J].激光生物学报,2014,(3):283-288,6.基金项目
临沂大学博士科研启动基金 （）
