激光生物学报Issue(6)：560-565,6.DOI:10.3969/j.issn.1007-7146.2014.06.009
鱼肉中磺胺类抗生素的表面增强拉曼光谱探测与分析
The Study of Sulfonamide Antibiotics in Fish Based on Surface-enhanced Raman Spectroscopy Technology
摘要
Abstract
In this paper,the silver colloid prepared by the microwave heating method was used as a Surface-enhanced Raman Scattering (SERS)active substrate for the trace-level detection of the sulfonamide antibiotics in fish.The extrac-tion effects of two extracts (ammonia water,ethyl acetate)had been compared.The results showed that the detection effect of ethyl acetate was better than ammonia water and the substances in fish had interference on the detection of anti-biotics.In this experiment,sulfamerazine and sulfamethazine were both detected with the lowest concentrations at 1 ppm by serving silver colloid as a SERS substrate and ethyl acetate as an extract,and the limits of detection were 0.16 ppm, 0.59 ppm,respectively.According to the results,the antibiotics in fish can be detected using SERS technology,and this work will provide an experimental foundation for the application of the detection of antibiotics in aquatic products.关键词
表面增强拉曼光谱/抗生素/银溶胶/氨水/乙酸乙酯Key words
Surface-enhanced Raman Spectroscopy/Antibiotics/Silver Colloid/Ammonia Water/Ethyl Acetate分类
数理科学引用本文复制引用
马海宽,韩晓红,张财华,张旭,史晓凤,马君..鱼肉中磺胺类抗生素的表面增强拉曼光谱探测与分析[J].激光生物学报,2014,(6):560-565,6.基金项目
国家自然科学基金青年基金项目（40906051）；中国博士后科学基金项目（2013M5330330）；山东省自然科学基金项目（BS2013HZ006）；青岛市科技计划基础研究项目（12-1-4-1-（1）-jch，14-2-4-96-jch） （）
