激光生物学报Issue(2)：152-157,6.DOI:10.3969/j.issn.1007-7146.2015.02.007
NADPH氧化酶参与 AtTOR调控盐胁迫诱导自噬机制的研究
Mechanism Study of NADPH Oxidase Involved in Regulation of Salt Stress-induced Autophagy through AtTOR in Arabidopsis thaliana
摘要
Abstract
Salinity seriously limits plant growth and development,reducing the crop productivity.Downregulation of cellular metabolism during salt stress causes reactive oxygen species (ROS)accumulation,contributing to oxidative stress and thus inducing autophagy.In this article we put emphasis on specific autophagic activity in plants during salt stress and its regulation mechanism.We found that autophagy was induced in the short term when treated with high salt concentrations,which was accompanied by a large increase in NADPH oxidase activity.In addition,we used LysoTrack-er Red (LTR)fluorescent probe as an indicator of autophagy to find that AtTOR inhibits the formation of autophagic body both in normal conditions and high salt stress conditions.After added with NADPH inhibitor DPI,autophagy was found significantly inhibited in WT during treatment with high concentrations of salt,while,no remarkable change was found in AtTOR mutant.Therefore NADPH oxidase is likely involved in the upstream regulation of AtTOR during salt stress-induced autophagy.The data may provide theoretic foundation for further studying the adaptive mechanisms and autophagic signaling pathway in plant.关键词
拟南芥/自噬/盐胁迫/AtTOR/NADPH氧化酶Key words
Arabidopsis thaliana/autophagy/salt stress/TOR/NADPH oxidase分类
生物科学引用本文复制引用
刘姿娴,刘建,王永强,曾礼漳,邢达..NADPH氧化酶参与 AtTOR调控盐胁迫诱导自噬机制的研究[J].激光生物学报,2015,(2):152-157,6.基金项目
国家高技术研究发展计划（863计划）课题（2007AA10Z204）；教育部“长江学者与创新团队计划”创新团队项目（IRT0829）；国家自然科学基金-广东联合基金重点项目 （）
