激光生物学报Issue(2)：180-185,6.DOI:10.3969/j.issn.1007-7146.2015.02.012
小球藻和莱茵衣藻原生质体的电转化研究
A Study on Electro-transformation in Protoplasts of Chlorella and Chlamydomonas
摘要
Abstract
The plasmid pCAMBIA1301 was transformed into The receptor cells Chlorella vulgaris and Chlamydomonas reinhardtii protoplasts by electroporation method.The related parameters was tested and molecular detections were per-formed in transformants.The results showed that:two kinds of algae were sensitive to hygromycin.The growth of Chlo-rella and Chlamydomonas was completely inhibited on solid media containing 25 mg/L and 100 mg/L concentration of hygromycin respectively.The optimal electric field intensity were 0.8 kV/cm and 0.6 kV/cm in Chlorella and Chlamydomonas respectively.The optimal pulse time was 10 ms for both species.Making protoplasts and treating with 2-deoxy-D-glucose make it possible to significantly increase the transformation rates.Molecular identification indicated that GUS gene has been successfully transformed into the two species of algae and the genetic form is stable.关键词
小球藻/莱茵衣藻/原生质体/电击转化Key words
Chlorella vulgaris/Chlamydomonas reinhardtii/protoplast/electroporation分类
生物科学引用本文复制引用
谢伟民,李合松,刘会珍,罗为桂,刘晓鹏,苏益..小球藻和莱茵衣藻原生质体的电转化研究[J].激光生物学报,2015,(2):180-185,6.基金项目
国家自然科学基金项目（No．91317312）；湖南省高校创新平台开放基金项目 （）
