井冈山大学学报（自然科学版）Issue(4)：44-47,4.DOI:10.3969/j.issn.1674-8085.2014.04.010
用于钝齿棒杆菌木糖代谢途径研究的基因工程菌构建
CONSTRUCTION OF GENETIC ENGINEERING STRAIN USED IN XYLOSE METABOLIC PATHWAY STUDY OF CORNEBACTERIUM CRENATUM
摘要
Abstract
Xylose isomerase gene(xylA)cloned from Escherichia coli genomic DNA by PCR was ligated to expression vector pXMJ19 to establish recombinant expression vectors pXMJ19-xylA, which was transformed to host strain Corynebacterium crenatum by electrotransformation method. Colony PCR and recombinant plasmids digested results showed that C. crenatum genetic engineering strain of harboring E.coli xylose isomerase gene has been constructed successfully. This study provides a foundation for producing succinic acid of C. crenatum xylose by sylose metabolic pathways and optimization of its metabolic pathway.关键词
钝齿棒杆菌/木糖异构酶基因/基因工程菌/转化Key words
Corynebacterium crenatum/xylose isomerase gene/genetic engineering strain/transformation分类
生物科学引用本文复制引用
张凤琴,刘俊,陈小举,晏娟..用于钝齿棒杆菌木糖代谢途径研究的基因工程菌构建[J].井冈山大学学报（自然科学版）,2014,(4):44-47,4.基金项目
安徽省高校学校省级自然科学项目(KJ2012Z270)；巢湖学院博士科研启动基金项目；巢湖学院科研项目(XLY-201208)。 （KJ2012Z270）
