井冈山大学学报（自然科学版）Issue(1)：40-45,6.DOI:10.3969/j.issn.1674-8085.2015.01.008
高产淀粉酶黑曲霉菌种诱变选育及固态发酵条件研究
MUTATION BREEDING AND SOLID-STATE FERMENTATION CONDITIONS OPTIMIZATION OF ASPERGILLUS NIGER WITH HIGH YIELD OFΑ-AMYLASE
摘要
Abstract
The fungal amylase has the characteristic of mild hydrolusis conditions and acid-resistance, and a wide range of application. By composite mutagenesis of microwave mutagenesis and diethyl sulfate treatment, isolate with high yield ofα-amylase was screened and the solid-state fermentation conditions were optimized. The results showed that, A. niger MD-17 was obtained after 16 s microwave mutagenesis and 20 min diethyl sulfate treatment. The optimal solid-state fermentation conditions followed as bran:corn flour=7:3;(NH4)2SO4:0.6%;initial pH 5;solid material:water 1:1.5. Fungalα-amylase enzyme activity could reached the highest 6011 U/g.关键词
黑曲霉/α-淀粉酶/联合诱变/固态发酵Key words
Aspergillus niger/α-amylase/mutation breeding/solid-state fermentation分类
化学化工引用本文复制引用
鲍勇阳,王露,王德培..高产淀粉酶黑曲霉菌种诱变选育及固态发酵条件研究[J].井冈山大学学报（自然科学版）,2015,(1):40-45,6.基金项目
天津市科技型中小企业技术创新项目(09ZXCXSH04400) （09ZXCXSH04400）
