江汉大学学报（自然科学版）Issue(5)：80-86,7.
豇豆SSR标记在豌豆中的可转移性与应用初探
Analysis and Application of Transferable SSR Molecular Markers from Cowpea(Vigna unguiculata)to Pea(Pisum sativum)
摘要
Abstract
Aimed to screen out transferable SSR(simple sequence repeat)molecular markers from cowpea (Vigna unguiculata) to pea (Pisum sativum). Using thirteen cowpea SSR primer pairs, eight primer pairs were successfully cross-amplified in pea. Five of the eight primer pairs exhibited polymorphism,and then to study the genetic variation of eleven pea cultivars and five cowpea culti⁃vars. Results showed that genetic parameters of the average number of alleles,the average effective number of alleles and the average Shannon′s index are 2. 00,1. 64,0. 55 in the pea cultivar group, and 2. 00,1. 80,0. 53 in the cowpea cultivar group,respectively. These indicated that a moderate level of genetic diversity was revealed in the two cultivar groups. In addtion,UPGMA clustering showed that the pea cultivar group and the cowpea cultivar group clustered into their own branches, respectively. Within each group,most of the samples can be distinguished from each other. Especial⁃ly,within the pea cultivar group,all the samples can be divided into two subgroups,which are con⁃sistent with their geographical origin.关键词
豌豆/豇豆/SSR/可转移性Key words
Pisum sativum/Vigna unguiculata/simple sequence repeat(SSR)/transferability分类
农业科技引用本文复制引用
潘磊,徐亚芳,郭瑞,李佳楠,胡志辉,陈禅友..豇豆SSR标记在豌豆中的可转移性与应用初探[J].江汉大学学报（自然科学版）,2014,(5):80-86,7.基金项目
湖北省自然科学基金项目（2013CBF213）；武汉市科技攻关项目（2013021001010478）；武汉市科技局青年晨光计划项目（2013071004010476）；湖北省豆类（蔬菜）植物工程技术研究中心开放基金项目（2014-10）；江汉大学高层次人才启动经费项目 （）
