江汉大学学报（自然科学版）Issue(6)：62-70,9.
外源性透明质酸减少R5- HIV对CD4+T细胞感染的研究
Exogenous Hyaluronic Acid Reduces R5- HIV Infection of CD4+T Cells
摘要
Abstract
Objective To study whether exogenous HA can reduce macrophage-tropic (CCR5-dependent, R5) HIV infectivity. Methods In this study,first using TZM-bl cell and un-stimulated CD4+ T cells assays to assess whether exogenous HA can reduce R5-HIV infectivity. Next treat the un-stimulated CD4+ T cells with hyaluroni⁃dase to study endogenous HA impact on R5-HIV infectivity. At last measures both exogenous HA, and endoge⁃nous HA effect on R5-HIV binding on CD4+ T cells. Results (1) 100 μg of exogenous HA treatment can reduce R5-HIV infectivity on TZM-bl cells and un-stimualted CD4+ T cells (P<0. 001). (2) Hyaluronidase treatment can enhance HIV infectivity, by adding 100μg of exogenous HA can reverse the function of hyaluronidase treatment(P<0. 001). (3) 100 μg of exogenous HA can reduce R5-HIV binding to CD4+ T cells, while hyaluronidase treatment can enhance R5-HIV binding to CD4+T cells (P<0. 001). Conclusion Exogenous HA can reduce R5-HIV in⁃fectivity on un-stimulated CD4+T cells, while hyaluronidase treatment can enhance R5-HIV binding to CD4+ T cells, and R5-HIV infectivity.关键词
透明质酸/R5-HIV/病毒感染/CD4+T细胞Key words
hyaluronic acid (HA)/R5-HIV/infectivity/CD4+T cell分类
医药卫生引用本文复制引用
李培林..外源性透明质酸减少R5- HIV对CD4+T细胞感染的研究[J].江汉大学学报（自然科学版）,2014,(6):62-70,9.基金项目
1R21AI104445-01A1(PL) （PL）
