中国当代医药Issue(18)：12-15,4.
体外共培养软骨细胞与脂肪基质细胞用于软骨构建的实验研究
Experimental study of in vitro co-culture of chondrocytes and adipose-de-rived stromal cells for cartilage construction
摘要
Abstract
Objective To investigate the feasibility of in vitro co-culture of chondrocytes and adipose-derived stromal cells (ADSCs) for cartilage construction. Methods ADSCs and porcine auricular chomdrocytes were collected and cul-tured in v itro,and then three groups were set as the experimental group,the positive control group and the negative con-trol group,which were inoculated ADSCs and chondrocytes(7:3 mixing ratio),simple chondrocytes,simply ADSCs respec-tively.And the contrast morphological changes,the wet weight,the proteoglycan content changes and type II collagen in the expression of histological feature of the three groups was observed and analyzed respectively. Results After eight weeks in v itro culture,the tissue of experimental group had a regular shape,which looked like the structure of cartilage tissue and was certain flexibility.For detection of the average wet weight and proteoglycan quantitative,the average wet weight and proteoglycan could reach 73.1%,81.9% of that in the positive experimental group respectively,which were significantly higher than that in the negative control group(P<0.01).HE staining showed that the experimental group oc-curred consecutive cartilage-like tissue,mature cartilage and fibrous tissue,and new cartilage thickness was more obvi-ous.Type II collagen immunohistochemical staining found that brownish yellow occurred near lacunas of cartilage in the experimental group. Conclusion Chondrocytes and ADSCs co-culture in vitro can be used to build cartilage,but further research is need to determine the direct evidence of ADSCs converted to mature chondrocytes.关键词
软骨细胞/脂肪基质细胞/共培养/软骨构建Key words
Chondrocytes/Adipose-derived stromal cells/Co-culture/Cartilage construction分类
医药卫生引用本文复制引用
贾黎,崔军..体外共培养软骨细胞与脂肪基质细胞用于软骨构建的实验研究[J].中国当代医药,2014,(18):12-15,4.基金项目
广东省深圳市知识创新计划基础研究项目 （）
