中国当代医药Issue(22)：20-22,3.
大鼠心室肌细胞急性酶分离及瞬时外向钾电流的记录
Separation of acute enzyme of ventricular myocyte and record of tran-sient outward potassium current in rats
张良胜 1李平 1许翔 1王莉 1范忠才2
作者信息
- 1. 河南省永城市人民医院心内科，河南永城 476600
- 2. 泸州医学院附属医院心内科，泸州 646000
- 折叠
摘要
Abstract
Objective To explore the method of acute isolate of separation of acute enzyme of ventricular myocyte and recording transient outward potassium current in adult SD rats. Methods Adult SD rats were selected as subjects, Lan-gendorff perfusion system and type II collagenase two step perfusion method were used to separate cell, and pacth clamp technique was used to record transient outward potassium current of ventricular myocyte in rats. Results The viable myocardium was seen Microscopically:it is lod or a rectangle,the surface is clean with clear cross striations and strong three-dimensional senseand it can record the transient outward potassium current up to 3000 pA. Conclusion Survival rate of cell of the method applied in the experiment and transient outward potassium current can be recorded.关键词
心肌细胞/瞬时外向钾电流/膜片钳技术Key words
Myocardial cell/Transient outward potassium current/Pacth clamp technique分类
医药卫生引用本文复制引用
张良胜,李平,许翔,王莉,范忠才..大鼠心室肌细胞急性酶分离及瞬时外向钾电流的记录[J].中国当代医药,2014,(22):20-22,3.
