中国当代医药Issue(24)：4-6,12,4.
siRNA沉默DNA聚合酶β基因表达对食管癌EC9706细胞放疗敏感性的影响
Effects of silencing DNA polymerase βby siRNA on radiotherapeutic sen-sitivity of esophageal carcinoma cell
摘要
Abstract
Objective To evaluate the effects of silencing DNA polymerase β by small interfering RNA (siRNA) on the radiotherapeutic sensitivity of human esophageal carcinoma cell line EC9706. Methods Various siRNA were transfect-ed into EC9706 cells with lipofectamine technique.Real-time PCR was performed to detect the expression levels of polβ mRNA in different groups,respectively.Three groups of cell were exposed to different doses of radiation (0,2,4,6,8 Gy).Cell survival ratio was detected by means of CCK8 method.And the detection of the apoptosis rate was completed by FCM. Results The expression of polβ mRNA and protein were surpressed obviously in the specific sequence siRNA group (P<0.01).The result of CCK8 showed that cell survival ratio was declining with the increase of radiotherapeutic dose (P<0.05).The cell apoptosis rates of silence polβ group was significantly higher than of not siRNA group and the blank control group (P<0.01),after irradiation,the cell apoptosis rate increase of silence polβ group was significantly higher than of not siRNA group and the blank control group (P<0.01). Conclusion The experiments confirm that si-lencing DNA polymeraseβby siRNA can significantly enhance the radiosensitivity of esophageal carcinoma cell.关键词
聚合酶β/siRNA/食管癌/放疗敏感性Key words
Polymeraseβ/siRNA/Esophageal carcinoma/Radiotherapeutic sensitivity分类
医药卫生引用本文复制引用
何婷玉,杜玉文,陈肖楠,赵国强..siRNA沉默DNA聚合酶β基因表达对食管癌EC9706细胞放疗敏感性的影响[J].中国当代医药,2014,(24):4-6,12,4.基金项目
国家自然科学基金 （）
