中国当代医药Issue(28)：24-26,3.
Th17细胞表达在丙种球蛋白耐药型川崎病发病机制中的作用研究
Effect study of Th17 cells expressing in the pathogenesis of gammaglobu-lin-resistant Kawasaki disease
摘要
Abstract
Objective To investigate effect of Th17 cells expressing in the pathogenesis of gammaglobulin-resistant Kawasaki disease. Methods 100 cases of children with Kawasaki disease in our hospital from May 2013 to May 2014 were selected as the research objects.According to whether the presence or absence of immunoglobulin resistance,100 patients were divided into gammaglobulin-resistant group and non-gammaglobulin-resistant group.30 normal children were selected as healthy control group.All patients were collected 4-5 ml peripheral blood and peripheral blood mononuclear cells were extrated.Th17 cells were detected by flow cytometry,IL-23P19 and RORrt gene were detected by mRNA fluorescence quantitative PCR expression. Results Percent of PBMC Th17 in CD4+cells of gammaglobulin-resistant group was significantly higher than that of non-gammaglobulin-resistant group and healthy control group re-spectively,percent of PBMC Th17 in CD4+ cells of non-gammaglobulin-resistant group was significantly higher than that of healthy control group,with statistical difference(P<0.05).RORrt gene expression of gammaglobulin-resistant group and non-gammaglobulin-resistant group was higher than that of healthy control group respectively (P<0.05).IL-23P19 mRNA expression had no statistical difference between non-gammaglobulin-resistant group and healthy control group (P>0.05). Conclusion Th17 cells expression and gammaglobulin-resistant Kawasaki disease was relevant.This provides a new approach for diagnosis and prognosis of immunoglobulin resistant Kawasaki disease,reduces cardiovascular injury sequelae,protects children’s physical and mental health,has a great social benefit,economic benefit.关键词
辅助性T淋巴细胞17/丙种球蛋白耐药型川崎病/发病机制Key words
Helper T lymphocytes 17/Gammaglobulin-resistant Kawasaki disease/Pathogenesis分类
医药卫生引用本文复制引用
康辉,罗丽娟,蔡素芬,邹峥..Th17细胞表达在丙种球蛋白耐药型川崎病发病机制中的作用研究[J].中国当代医药,2014,(28):24-26,3.基金项目
江西省卫生厅科技计划项目 （）
