中国当代医药Issue(7)：4-7,4.
七氟醚预处理抑制TNF-α诱导的血管内皮细胞ICAM-1表达与JNK的相关性研究
The role of JNK in sevoflurane preconditioning inhibits TNF-α induced ICAM-1 expression in vascular endothelial cells
摘要
Abstract
Objective To investigate the role of c-Jun N-terminal kinase (JNK) in sevoflurane preconditioning inhibits tumor necrosis factor-α (TNF-α) induced intercellular adhesion molecule-1 (ICAM-1) expression in vascular endothe-lial cells. Methods Human umbilical vein endothelial cells were randomly divided into 5 groups with 3 wells in each group:normal control group (group C),TNF-α treatment group (group T),sevoflurane preconditioning group (group S), sevoflurane preconditioning and TNF-α treatment group (group S+T),JNK inhibitor SP600125 and sevoflurane precon-ditioning and TNF-α treatment group (group SP+S+T).1.5MAC sevoflurane saturated buffer was added to the culture medium and the cells underwent 3 episodes of 15 min incubation with sevoflurane at 15 min interval in group S,group S+T and group SP+S+T.The cells were incubated with SP600125 (30 μmol) 1 h before sevoflurane preconditioning in group SP+S+T.Human umbilical vein endothelial cells were treated with TNF-α (10 ng/ml) in group T,group S+T and group SP+S+T 40 min after the end of sevoflurane preconditioning.The expression level of ICAM-1 protein and the phosphorylation level of JNK were measured by Western blot. Results Compared with group C,the expression level of ICAM-1 and the phosphorylation level of JNK were activated by TNF-α in group T and group S+T (P<0.05),but was not significant in group S (P>0.05).Compared with group T,sevoflurane pretreatment could decrease the expression level of ICAM-1 and p-JNK in group S+T (P<0.05).Compared with group S+T,ICAM-1 and p-JNK expression was down-regulated in group SP+S+T (P<0.05). Conclusion The reduction of p-JNK expression plays a role in sevoflurane pre-conditioning inhibits TNF-αinduced ICAM-1 expression in vascular endothelial cells.关键词
七氟醚/血管内皮细胞/细胞间黏附分子-1/c-Jun氨基末端激酶Key words
Sevoflurane/Vascular endothelial cell/Intercellular adhesion molecule-1/c-Jun N-terminal kinase分类
医药卫生引用本文复制引用
闫华磊,李静静,李睿,赵建力..七氟醚预处理抑制TNF-α诱导的血管内皮细胞ICAM-1表达与JNK的相关性研究[J].中国当代医药,2015,(7):4-7,4.基金项目
国家自然科学基金资助项目 （）
