中国当代医药Issue(12)：26-29,4.
甲泼尼松龙治疗大鼠脊髓损伤的行为学和组织病理学研究
Research on behavioristics and histopathology of methylprednisolone in the treatment of rat with spinal cord injury
摘要
Abstract
Objective To study effect on behavioristics and histopathology of methylprednisolone in the treatment of rat with spinal cord injury. Methods Sixty SD male rats were randomly divided into three groups,each group had 20 rats. Group A was normal control group,group B was injury control group,and group C was treatment group.Rats’ spine in group A was merely respected by total laminectomy without any hurts to its spinal cord.Both group B and C were as-signed to be evaluated by using spinal cord injury model (T10) made by Allen method.Rats in group C were injected methylprednisolone which amounted to 30 mg/kg at the beginning.However,the remalning were injected at the amount of 5.4 mg/kg each time,23 hours to the end.Methylprednisolone was replaced by normal saline instead in group A and B,the same dose of group C.After operation of 1 day,1 week,2 weeks and 4 weeks,the hind limb motor function includ-ing BBB score and inclined plane test among three groups were applied.After operation of 1 day and 4 weeks,injured spinal cord of 10 rats were observed under microscopy after HE-stalning. Results BBB score and inclined plane test angle in group C was significantly higher than that in group B at each time point respectively,with statistical difference (P<0.01).Histopathological study showed that hemorrhage and edema in the spinal cord after injury,and vacuolation in cells,nervous tissue melted and demyelination changed.Pathological changed of group C was lighter than that in group B. Conclusion Methylprednisolone can restraln inflammatory response of spinal cord injury,reduce range of cavity and tissue necrotic,improve function of behavior.关键词
甲泼尼松龙/脊髓损伤/行为学/组织病理学Key words
Methylprednisolone/Spinal cord injury/Behavioristics/Histopathology分类
医药卫生引用本文复制引用
董乐乐,郭鹏年,于泽..甲泼尼松龙治疗大鼠脊髓损伤的行为学和组织病理学研究[J].中国当代医药,2015,(12):26-29,4.基金项目
内蒙古自治区自然科学基金资助项目 （）
