中国当代医药Issue(14)：7-10,4.
α27基因特异性siRNA对单纯疱疹病毒Ⅱ型感染宿主细胞的影响
Effect of α27-specific siRNA on host cells infecting by herpes simplex virus typeII
摘要
Abstract
Objective To determine the effect of α27-specific siRNA on host cells infecting by herpes simplex virus typeII (HSV-II). Methods Fourα27-specific siRNAs (R1,R2,R3,R4 group) and the positive,negative control siRNAs were synthesized in vitro by chemical process.The Vero cells were transfected with siRNAs using Lipofectamine2000 followed by HSV-IIinfecting.The activity of the cells were measured at 12,24,36,48,60,72 h by MTT. Results Groups with α27-specific siRNA had higher OD values by MTT colorimetric assay than blank cells,with better survive situa-tion.R2 and R4 group had better survival than other groups. Conclusion α27-specific siRNA can protect host cells of HSV-II.There are obvious differences of inhibition effect of siRNA which are synthesized in accordance with the same priciples.关键词
α27基因/siRNA/RNA干扰/单纯疱疹病毒Ⅱ型/Vero细胞Key words
α27 gene/siRNA/RNAi/HSV-II/Vero cell分类
生物科学引用本文复制引用
杨兆林,陈茵,汪莉,赵少锋,邵艳玲..α27基因特异性siRNA对单纯疱疹病毒Ⅱ型感染宿主细胞的影响[J].中国当代医药,2015,(14):7-10,4.基金项目
广东省科技计划项目 （）
