吉林农业大学学报Issue(2)：149-152,170,5.DOI:10.13327/j.jjlau.2014.1733
人参鲨烯环氧酶基因的克隆与序列分析
Cloning and Sequence Analysis of Squalene Epoxidase Gene in Panax gin-seng
摘要
Abstract
Five-year ginseng root tissue was used as material .After extracting the total RNA and revers-ing transcription,Squalene Epoxidase (SQE)gene was cloned and sequenced by RT-PCR technology . Then the impact factors of ginseng saponins′biosynthetic pathway was discussed .The results showed that Panax ginseng SQE was cloned successfully,with a full length of 1 636 bp,containing a 1 61 1 bp open reading frame that encode 536 amino acids .The nucleotide showed a high homology with other plants . Furthermore,phylogenic analysis on the sequence of SQE gene with other plants showed that P .ginseng was closely related to P .Notoginseng,Alalia elata and Eleutherococcus senticosus,and the identities of nucleotide sequences reached 98%,96%and 90%,respectively .关键词
人参皂苷/鲨烯环氧酶基因/克隆/序列分析/生物合成/RT-PCRKey words
ginsenoside/SQE gene/cloning/sequence analysis/biosynthesis/RT-PCR分类
农业科技引用本文复制引用
侯双利,刘翠晶,杨利民,韩梅..人参鲨烯环氧酶基因的克隆与序列分析[J].吉林农业大学学报,2014,(2):149-152,170,5.基金项目
国家自然科学基金项目 （）
