吉林农业大学学报Issue(3)：276-281,6.DOI:10.13327/j.jjlau.2014.1919
人参黑斑病拮抗细菌的分离筛选和鉴定
Screening and Identification of Antagonistic Bacteria on Alternaria panax Whetz
摘要
Abstract
Using the plate confrontation assay ,fifteen strains with antagonistic effect on Alternaria panax Whetz were screened out of 113 bacteria isolated from the ginseng rhizosphere soil of Wanliang region of Jilin province .The plate-confrontation method and the growth speed rate method were used for preliminary screening and antimicrobial test in vitro .Pot trials were carried out to assess the inhibitory activity of strain ND-60 in vivo .The results showed that the inhibition rate of strain ND-60 fermentation broth was 82.43% to A .p .Whetz and also had good broad-spectrum antimicrobial effect .The strain ND-60 had significant control efficacy ( P<0 .05 ) when compared with the pesticides treatments ,and the protective and therapeutic efficacy of the aseptic filtrate of ND-60 reached 80.21% and 72.22% ,respectively .Ac-cording to morphologic , physiological and biochemical characteristic identification and 16S rDNA se-quence analysis ,strain ND-60 was classified as Bacillus amyloliquefaciens .关键词
人参黑斑病/解淀粉酶芽孢杆菌/生物防治Key words
Alternaria panax Whetz/Bacillus amyloliquefaciens/biocontrol分类
农业科技引用本文复制引用
孙卓,杨利民,马秀杰,张一鸣..人参黑斑病拮抗细菌的分离筛选和鉴定[J].吉林农业大学学报,2014,(3):276-281,6.基金项目
国家科技支撑计划项目（2011BAI03B01-02），国家自然科学基金项目（31270371），吉林省科技发展计划重点项目 （）
