吉林农业大学学报Issue(2)：191-196,6.DOI:10.13327/j.jjlau.2014.2122
牛GPAM基因分子克隆及编码区E1－76－C＞T定点突变前后生物信息学对比分析∗
Cloning of Bovine GPAM Gene and Comparative Analysis of Bioinfor-matics Before and After Coding Region E1-76-C> T Site-Specific Mutagenesis
摘要
Abstract
The main function of Mitochondrial glycerol⁃3⁃phosphate acyltransferase( GPAM) gene is to promote production of triglyceride in animal body through catalytic triacylglycero phospholipid bio⁃synthesis, and to regulate fat deposition, energy consumption, carcass weight and overall metabolism of mammals through central and peripheral regulating action. In this study, coding region of bovine GPAM gene was amplified via specific primers by PCR method, and was cloned and sequenced. Bioinformatics methods were used for comparatively analyzing physical and chemical properties, structure and function between mutant type and normal type, assuming that mutation occurred withE1-76-C>T site of bovine GPAM gene coding sequence. Compartive analysis of bioinformatics showed that bovine GPAM gene single nucleotide change can affect amino acid coding, and there are significant differences in primary structure, secondary structure and tertiary structure of protein.关键词
牛/GPAM基因/编码区克隆/定点突变/生物信息学Key words
bovine/GPAM gene/cloning of coding region/site-specific mutagenesis/bioinformat-ics分类
农业科技引用本文复制引用
于海滨,李傲楠,张晓军,赵志辉,于仙忠,芦春艳..牛GPAM基因分子克隆及编码区E1－76－C＞T定点突变前后生物信息学对比分析∗[J].吉林农业大学学报,2015,(2):191-196,6.基金项目
国家“863”计划项目 （）
