吉林农业大学学报Issue(2)：197-202,6.DOI:10.13327/j.jjlau.2015.2520
体细胞克隆牛生产效率的影响因素∗
Factors Affecting the Production of Cloned Cattle by Somatic Cell Nu-clear Transfer
摘要
Abstract
The production of cloned cattle by somatic cell nuclear transfer ( SCNT ) is affected by many factors both in laboratory and embryo transfer. Based on our experimental data in recent years, factors affecting the production of cloned cattle by SCNT such as embryo transfer numbers, season changes and physiological conditions of surrogate cattle, etc, were analyzed in this study. The results showed that: ( 1 ) Compared with one⁃or three⁃embryo transfer, two⁃embryo transfer could obtain relatively higher pregnancy rate, lower abortion rate and higher calving rate. (2) The efficiency of embryo transfer in spring, summer and autumn ( from May to September) was significantly better than that of winter (from December to January). (3) The pregnancy rate of surrogate cattle with A and B rank corpus luteum is significantly higher than that with C rank. The results indicate that num⁃ber of transferred embryos, seasonal changes and physiological conditions of surrogate cattle have acritical impact on the production of cloned cattle by SCNT. This study will provide theoretical and experimental basis for further optimization of cloned cattle production program by SCNT, and then improve the efficiency of cloned cattle production.关键词
牛/克隆/体细胞核移植/胚胎移植Key words
cattle/cloning/somatic cell nuclear transfer/embryo transfer分类
农业科技引用本文复制引用
王爱兵,张胜,王金柱,李武,唐博,张学明,李子义..体细胞克隆牛生产效率的影响因素∗[J].吉林农业大学学报,2015,(2):197-202,6.基金项目
国家转基因生物新品种培育专项 （）
