吉林农业大学学报Issue(2)：216-220,5.DOI:10.13327/j.jjlau.2014.2105
结核分枝杆菌保护性抗原 TB10�4基因的克隆和真核表达载体的构建∗
Cloning of Mycobacterium Tuberculosis Protective Antigen TB10 4 Gene and Construction of Eukaryotic Expression Vector
摘要
Abstract
Mycobacterium tuberculosis protective antigen TB10�4 belongs to ESAT⁃6 family of Myco⁃bacterium tuberculosis early⁃secreted protein, and it works equally or better in immunogenicity than ESAT⁃6. In this study, the gene was cloned by molecular biological technique. Compared with the published TB10�4 gene sequence in GenBank ( NP_214802�1) , their homology was found as high as 100. Then it was connected with eukaryotic expression vector pEGFP⁃N1 to build recombinant plasmid pEGFP⁃N1⁃TB10�4. The study provides experimental data for application of TB10�4 gene in Mycobacterium tuberculosis diagnosis and vaccine antigens.关键词
结核分枝杆菌/早期分泌蛋白/克隆/真核表达载体Key words
Mycobacterium tuberculosis/early-secreted protein/cloning/eukaryotic expression vec-tor分类
生物科学引用本文复制引用
吕永超,张文慧,张林波..结核分枝杆菌保护性抗原 TB10�4基因的克隆和真核表达载体的构建∗[J].吉林农业大学学报,2015,(2):216-220,5.基金项目
吉林省科技发展计划项目 （）
