吉林农业大学学报Issue(2)：226-231,236,7.DOI:10.13327/j.jjlau.2015.2493
微小隐孢子虫TSP3蛋白质的表达与功能
Expression and Function of Cryptosporidium parvum TSP3 Protein
摘要
Abstract
By cloning Cryptosporidium parvum TSP3, prokaryotic expression vector was constructed, and to immunize New Zealand rabbits, TSP3⁃His tag of recombinant protein was purified. By indirect ELISA method, specific serum titers were detected and polyclonal antibodies were purified. The pu⁃rified TSP3⁃GST tag of recombinant protein was incubated with HCT⁃8 cells by Western blot and in⁃direct ELISA cell adhesion properties. This study successfully constructed Cryptosporidium parvum pET⁃TSP3 and pGEX⁃TSP3 recombinant plasmid, and by affinity chromatography successfully puri⁃fied recombinant proteins of high quality. CpTSP3 recombinant proteins are of good immunogenicity and can bind HCT⁃8.关键词
微小隐孢子虫/TSP3/原核表达/多克隆抗体/细胞黏附Key words
Cryptosporidium parvum/TSP3/prokaryotic expression/polyclonal antibody/cell ad-hesion分类
农业科技引用本文复制引用
孙婷,张星星,余胜超,刘刚,尹继刚..微小隐孢子虫TSP3蛋白质的表达与功能[J].吉林农业大学学报,2015,(2):226-231,236,7.基金项目
国家自然科学基金项目 （）
