吉林中医药Issue(7)：719-721,722,4.DOI:10.13463/j.cnki.jlzyy.2015.07.022
培育颗粒对人绒毛滋养细胞侵袭与增殖的作用
Influence of the Peiyu particles on invasion and proliferation of human trophoblast cell
摘要
Abstract
Objective To observe the Effect of the Peiyu particles on invasion and proliferation capabilities of human tro-phoblast cell .Methods Placental villi from normal pregnancy of 5 to 10 weeks were taken to culture trophoblast cells . Twenty percent pharmacological serum obtained from Peiyu particles-administered normal female SD rats (aged 23 to 25 days) was added in nutrient solution for culture .After being divided into groups ,trophoblast cells were cultured for 48 hours .Then ,invasion cell number ,absorbance and PCNA expression rate of trophoblast cell in all groups were analyzed by TW invasion assay ,MTT assay ,and flow cytometry ,respectively .Results TW assay results show that invasion cell number of each dose group of Peiyu particles is different from that of the normal group ,and the invasion cell number of medium dose group is the highest .MTT assay results show that each dose group of Peiyu particles is different from the normal group in ab-sorbance with absorbance of medium dose group being the highest .Flow cytometry results also show that there are differences between each dose group of Peiyu particles and the normal group in PCNA expression rate ,and the PCNA expression rate of medium dose group is the highest .Conclusion Peiyu particles can enhance invasion and proliferation abilities of human trophoblast cell ,and its effect may be dose-dependent .关键词
培育颗粒/侵袭/增殖/滋养细胞/负反馈调节Key words
Peiyu particles/invasion/proliferation/trophoblast cell/negative feedback to adjust分类
医药卫生引用本文复制引用
王婧瑶,孙丽萍,赵丕文,梁欣韫..培育颗粒对人绒毛滋养细胞侵袭与增殖的作用[J].吉林中医药,2015,(7):719-721,722,4.基金项目
国家自然科学基金青年基金项目（81001584）；北京市自然科学基金面上项目（7112053）。 （）
