解剖学杂志Issue(3)：272-274,390,4.DOI:10.3969/j.issn.1001-1633.2015.03.004
大鼠视网膜发育过程中细胞增殖与凋亡的形态学
Morphological research on proliferation and apoptosis in rat retina development❋
摘要
Abstract
Obj ective :To observe the change of proliferating cell nuclear antigen (PCNA)expression and cell apoptosis during the retina development of rats.Methods :Rats from embryonic days 14-20 (E14-20),postnatal days 0-1 5 (P0-1 5 )and adult (P36)were used.The eyeballs were removed and embedded in paraffin for immunohistochemical staining and terminal deoxynucleotidyl transferase-mediated dUTP nick end labeling (TUNEL).Results :PCNA was firstly expressed in large quantities in rat retina from E14 to P7,decreased obviously since P9,and could not be observed since P1 5 .Apoptotic cells appeared in small quantities since E18.And then apoptotic cells increased dramatically with the retina development,and reached the peak at P7.As maturation continued,these cells decreased gradually.Apoptotic cells could not be seen in the mature retina.Conclusion:Orderly and dynamic changes of cell proliferation and apoptosis were detected during the retina development,which was essential for the development of normal structure and function of the retina.关键词
视网膜/发育/增殖细胞核抗原/增殖/凋亡Key words
retina/development/proliferating cell nuclear antigen/proliferation/apoptosis引用本文复制引用
游庆朋,史为博,刘洁,齐倩,李英敏,左敏,丛斌..大鼠视网膜发育过程中细胞增殖与凋亡的形态学[J].解剖学杂志,2015,(3):272-274,390,4.基金项目
河北省自然科学基金(H2013206138) （H2013206138）
