寄生虫与医学昆虫学报Issue(1)：6-16,11.DOI:10.3969/j.issn.1005-0507.2014.01.002
rDNA-ITS2序列作为中国按蚊分子鉴别特征的评述：Ⅱ塞蚊亚属
THE SECOND INTERNAL TRANSCRIBED SPACER OF NUCLEAR RIBOSOMAL DNA AS A MOLECULAR IDENTITY FOR CHINESE ANOPHELINE TAXONOMY:Ⅱ SUBGENUS CELLIA SPECIES
摘要
Abstract
To evaluate the ribosomal DNA second internal transcribed spacer (rDNA-ITS2) sequences as an identification marker of genus Cellia Subgenus Anopheles mosquito species in China.The rDNA-ITS2 sequences of subgenus Cellia mosquito species in China registered in the GenBank database were downloaded and analysized .The intraspecific variation and interspecific difference ( p-distance ) were estimated by MEGA software.By July 2013, there were 442 of ITS2 sequences of the subgenus Cellia registered on GenBank, which belong to 27 species.The value of intraspecific p-distance was less than 1%in most species.The species-specific ITS2 sequences of An.subpictus, An.fluviatilis and An.indefinitus were unable to determine due to larger differences within species.To ignore the above three species, the range of interspecific p-distance among other species was from 2.80%( An.dirus with An.dirus D) to 68.3%( An.maculates with An.vagus) .The results suggested that the rDNA-ITS2 sequence has a good conservation and interspecific resolution in most of the Cellia mosquitoes but cannot be used to distinguish several species and forms.关键词
rDNA-ITS2/塞蚊亚属/鉴别/评述Key words
rDNA-ITS2/subgenus Cellia/taxonomy/review引用本文复制引用
伍桐,诸德源,王一然,马雅军..rDNA-ITS2序列作为中国按蚊分子鉴别特征的评述：Ⅱ塞蚊亚属[J].寄生虫与医学昆虫学报,2014,(1):6-16,11.基金项目
国家科技重大专项课题（2012ZX10004219-010）；2012年和2013年度第二军医大学大学生创新能力培养基金项目 （）
