寄生虫与医学昆虫学报Issue(4)：263-267,5.DOI:10.3969/j.issn.1005-0507.2014.04.009
艾美耳球虫子孢子从宿主细胞中逸出机制的初步研究
PRELIMINARY STUDY ON THE MECHANISMS OF RAPID EGRESS OF EIMERIA TENELLA SPOROZOITES FROM HOST CELLS
摘要
Abstract
Apicomplexan parasites infect nearly all vertebrate hosts even including humans and cause some severe diseases such as malaria, toxoplasmosis, cryptosporidiosis which are responsible for substantial economic losses. Like most intracellular pathogens, egress from host cells is a vital step of the apicomplexan parasites, which attracted attentions of many research groups.Unfortunately, as an important genus of Phylum Apicomplexa, little information of egress is known on Eimeria species which leads large amount of losses to poultry industry annually.In this report we used ethanol to induce egress of E.tenella M2e transgenic strain (EtM2e) sporozoites which express yellow fluorescent protein (YFP).Results showed that ethanol could induce egress of EtM2e sporozoites from infected Madin Derby Bovine Kidney ( MDBK) cells, and this process was depended on the mobility of the parasites.We also found that ethanol could also stimulate microneme protein discharge.Furthermore, both the course of egress and the secretion of microneme were controlled by the flux of intracellular Ca2+of the parasite.Taken together, our results preliminarily explained the mechanism of egress of eimerian parasites, which provided clues to the further study.关键词
柔嫩艾美耳球虫/子孢子/乙醇/逸出Key words
Eimeria tenella/Sporozoites/Ethanol/Egress引用本文复制引用
闫鑫磊,计永胜,田秀玲,索静霞,刘贤勇,索勋..艾美耳球虫子孢子从宿主细胞中逸出机制的初步研究[J].寄生虫与医学昆虫学报,2014,(4):263-267,5.基金项目
国家自然科学基金项目 （）
