江苏大学学报（医学版）Issue(6)：474-477,4.DOI:10.13312/j.issn.1671-7783.y140187
H3受体激动剂对变应性鼻炎豚鼠鼻黏膜嗜酸性粒细胞浸润及嗜酸性粒细胞趋化因子含量的影响
Effect of H3 receptor agonist on eosinophils and eotaxin of allergic rhinitis in guinea pigs
摘要
Abstract
Objective:To explore the influence of histamine receptor 3 (H3 R)agonist,IMETIT,on eo-sinophils and one of eosinophilic chemokines (eotaxin)in allergic rhinitis(AR)model using guinea pigs. Methods:Thirty guinea pigs were randomly divided into three groups:control group,sensitized group with-out treatment and sensitized group with drug treatment.The sensitized group with or without drug treatment were immunized by ovalbumin (OVA)to establish AR model and the control group by saline to contrast, then they were stimulated intranasal by ovalbumin (1 %)respectively.The times of sneezing and scratching nose in 30 min were recorded.The control group and the sensitized group without treatment were given gas-tric gavage by saline.And the sensitized group with drug treatment were given gastric gavage by saline (1 0 mL/kg)with IMETIT (30 mg/kg).Then we investigated the eosinophil infiltration degrees and eosinophilc chemokines content in nasal septum mucosa using HE staining and immunohistochemistry technology.Re-sults:There was significant difference between the control group and the sensitized group with or without drug treatment in the numbers of sneezing and the numbers of scratching nose(P =0.000).There was no significant difference between the sensitized group with or without drug treatment in the means of eosinophil and eotaxin positive expression rate in nasal septum mucosa(P =0.581 ,0.676).Conclusion:The eosin-ophil infiltration and the level of eotaxin of nasal septum mucous could not be reduced by histamine H3 re-ceptor agonist,IMETIT.关键词
H3受体激动剂/变应性鼻炎/嗜酸性粒细胞/嗜酸性粒细胞趋化因子Key words
H3 receptor agonist/allergic rhinitis/eosinophil/eotaxin分类
医药卫生引用本文复制引用
窦毅,杨旭东,黄秋生..H3受体激动剂对变应性鼻炎豚鼠鼻黏膜嗜酸性粒细胞浸润及嗜酸性粒细胞趋化因子含量的影响[J].江苏大学学报（医学版）,2014,(6):474-477,4.基金项目
江苏大学医学临床科技发展基金资助项目 （）
